Heinl, Elena -Sofia and Lorenz, Sebastian and Schmidt, Barbara and Laqtom, Nouf Nasser M. and Mazzulli, Joseph R. and Francelle, Laetitia and Yu, Timothy W. and Greenberg, Benjamin and Storch, Stephan and Tegtmeier, Ines and Othmen, Helga and Maurer, Katja and Steinfurth, Malin and Witzgall, Ralph and Milenkovic, Vladimir and Wetzel, Christian H. and Reichold, Markus (2022) CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry. ISCIENCE, 25 (10): 105082. ISSN 2589-0042
Full text not available from this repository. (Request a copy)Abstract
The SARS-CoV-2 virus has triggered a worldwide pandemic. According to the BioGrid database, CLN7 (MFSD8) is thought to interact with several viral pro-teins. The aim of this work was to investigate a possible involvement of CLN7 in the infection process. Experiments on a CLN7-deficient HEK293T cell line ex-hibited a 90% reduced viral load compared to wild-type cells. This observation may be linked to the finding that CLN7 ko cells have a significantly reduced GM1 content in their cell membrane. GM1 is found highly enriched in lipid rafts, which are thought to play an important role in SARS-CoV-2 infection. In contrast, overexpression of CLN7 led to an increase in viral load. This study provides evi-dence that CLN7 is involved in SARS-CoV-2 infection. This makes it a potential pharmacological target for drug development against COVID-19. Furthermore, it provides insights into the physiological function of CLN7 where still only little is known about.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEURONAL CEROID-LIPOFUSCINOSIS; LIPID RAFTS; MEMBRANE GLYCOPROTEIN; CHOLERA-TOXIN; GANGLIOSIDES; CHOLESTEROL; GM1; MUTATIONS; BIOSYNTHESIS; ENDOCYTOSIS |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Psychiatrie und Psychotherapie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Ralph Witzgall |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Jan 2024 05:22 |
| Last Modified: | 30 Jan 2024 05:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57217 |
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