Klein, Silvan and Siegmund, Andreas and Eigenberger, Andreas and Hartmann, Valerie and Langewost, Felix and Hammer, Nicolas and Anker, Alexandra and Klein, Konstantin and Morsczeck, Christian and Prantl, Lukas and Felthaus, Oliver (2022) Peripheral Nerve Regeneration-Adipose-Tissue-Derived Stem Cells Differentiated by a Three-Step Protocol Promote Neurite Elongation via NGF Secretion. CELLS, 11 (18): 2887. ISSN , 2073-4409
Full text not available from this repository. (Request a copy)Abstract
The lack of supportive Schwann cells in segmental nerve lesions seems to be one cornerstone for the problem of insufficient nerve regeneration. Lately, adipose-tissue-derived stem cells (ASCs) differentiated towards SC (Schwann cell)-like cells seem to fulfill some of the needs for ameliorated nerve recovery. In this study, three differentiation protocols were investigated for their ability to differentiate ASCs from rats into specialized SC phenotypes. The differentiated ASCs (dASCs) were compared for their expressions of neurotrophins (NGF, GDNF, BDNF), myelin markers (MBP, P0), as well as glial-marker proteins (S100, GFAP) by RT-PCR, ELISA, and Western blot. Additionally, the influence of the medium conditioned by dASCs on a neuron-like cell line was evaluated. The dASCs were highly diverse in their expression profiles. One protocol yielded relatively high expression rates of neurotrophins, whereas another protocol induced myelin-marker expression. These results were reproducible when the ASCs were differentiated on surfaces potentially used for nerve guidance conduits. The NGF secretion affected the neurite outgrowth significantly. It remains uncertain what features of these SC-like cells contribute the most to adequate functional recovery during the different phases of nerve recovery. Nevertheless, therapeutic applications should consider these diverse phenotypes as a potential approach for stem-cell-based nerve-injury treatment.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REPROGRAMS SCHWANN-CELLS; MARROW STROMAL CELLS; NEUROTROPHIC FACTORS; FUNCTIONAL RECOVERY; RECEPTOR; LAMININ; EXPRESSION; PHENOTYPE; OUTGROWTH; NEURONS; adipose-tissue-derived stem cells; Schwann cells; nerve regeneration; neural differentiation; neurotrophic factors |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Feb 2024 07:33 |
| Last Modified: | 14 Feb 2024 07:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57275 |
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