Muehler, Denise and Mao, Xiaojun and Czemmel, Stefan and Geissert, Janina and Engesser, Christina and Hiller, Karl-Anton and Widbiller, Matthias and Maisch, Tim and Buchalla, Wolfgang and Al-Ahmad, Ali and Cieplik, Fabian (2022) Transcriptomic Stress Response in Streptococcus mutans following Treatment with a Sublethal Concentration of Chlorhexidine Digluconate. MICROORGANISMS, 10 (3): 561. ISSN , 2076-2607
Full text not available from this repository. (Request a copy)Abstract
Despite the widespread use of antiseptics such as chlorhexidine digluconate (CHX) in dental practice and oral care, the risks of potential resistance toward these antimicrobial compounds in oral bacteria have only been highlighted very recently. Since the molecular mechanisms behind antiseptic resistance or adaptation are not entirely clear and the bacterial stress response has not been investigated systematically so far, the aim of the present study was to investigate the transcriptomic stress response in Streptococcus mutans after treatment with CHX using RNA sequencing (RNA-seq). Planktonic cultures of stationary-phase S. mutans were treated with a sublethal dose of CHX (125 mu g/mL) for 5 min. After treatment, RNA was extracted, and RNA-seq was performed on an Illumina NextSeq 500. Differentially expressed genes were analyzed and validated by qRT-PCR. Analysis of differential gene expression following pathway analysis revealed a considerable number of genes and pathways significantly up- or downregulated in S. mutans after sublethal treatment with CHX. In summary, the expression of 404 genes was upregulated, and that of 271 genes was downregulated after sublethal CHX treatment. Analysis of differentially expressed genes and significantly regulated pathways showed regulation of genes involved in purine nucleotide synthesis, biofilm formation, transport systems and stress responses. In conclusion, the results show a transcriptomic stress response in S. mutans upon exposure to CHX and offer insight into potential mechanisms that may result in development of resistances.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHYSIOLOGICAL SIGNIFICANCE; PHOSPHOTRANSFERASE SYSTEM; MALOLACTIC FERMENTATION; ANTIMICROBIAL AGENTS; PURINE METABOLISM; EXTRACELLULAR DNA; ORAL STREPTOCOCCI; ALKALI PRODUCTION; BIOFILM; RESISTANCE; antibacterial; CHX; RNA-seq; Streptococcus mutans; stress response; transcriptomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Feb 2024 07:56 |
| Last Modified: | 14 Feb 2024 07:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57285 |
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