Gallorini, Marialucia and Widbiller, Matthias and Bolay, Carola and Carradori, Simone and Buchalla, Wolfgang and Cataldi, Amelia and Schweikl, Helmut (2022) Relevance of Cellular Redox Homeostasis for Vital Functions of Human Dental Pulp Cells. ANTIOXIDANTS, 11 (1): 23. ISSN , 2076-3921
Full text not available from this repository. (Request a copy)Abstract
Odontogenic MSCs are vulnerable to LPS-triggered bacterial infections, and they respond by secreting inflammatory mediators, such as IL-6, and with mineralization. Since both processes might be prone to a disturbance of the redox homeostasis, the oxidative stress influence on vital functions of human dental pulp cells (HPCs) was investigated. With these aims, a model of LPS-stimulated primary HPCs was established, and anti- and pro-oxidant substances were administered up to 21 days to measure inflammation and mineralization parameters. LPS-stimulated HPCs retained mineralization potential, which was decreased with the antioxidants NAC and fisetin and the pro-oxidant BSO. The expression of surface markers related to odontogenic commitment was influenced accordingly but counteracted by the enhanced expression of BMP2 and ALP at the transcriptional level. LPS triggers an early IL-6 production in non-odontogenic conditions, while it can be measured only after 15 days in the presence of the differentiation medium. The present study shows that HPCs functions causally depend on a tightly regulated cellular redox balance. Our data demonstrate a redox control of pulp MSC odontogenic commitment along with a potential association between an IL-6 late secretion and mineralization. These findings lay the groundwork for investigations on the molecular role of IL-6 in dental hard tissue metabolism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; INDUCED OXIDATIVE STRESS; OSTEOGENIC DIFFERENTIATION; IL-6; ACID; IL-6; oxidative stress; inflammation; mineralization; dental pulp; fisetin; LPS; immunophenotype; BMP2; ALP |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Prof. Dr. rer. nat. Helmut Schweikl |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Feb 2024 10:38 |
| Last Modified: | 14 Feb 2024 10:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57383 |
Actions (login required)
 |
View Item |
