Hochheiser, Inga and Pilsl, Michael and Hagelueken, Gregor and Moecking, Jonas and Marleaux, Michael and Brinkschulte, Rebecca and Latz, Eicke and Engel, Christoph and Geyer, Matthias (2022) Structure of the NLRP3 decamer bound to the cytokine release inhibitor CRID3. NATURE, 604 (7904). pp. 184-189. ISSN 0028-0836, 1476-4687
Full text not available from this repository. (Request a copy)Abstract
NLRP3 is an intracellular sensor protein that when activated by a broad spectrum of exogenous and endogenous stimuli leads to inflammasome formation and pyroptosis(1,2). The conformational states of NLRP3 and the way antagonistic small molecules act at the molecular level remain poorly understood(2,3). Here we report the cryo-electron microscopy structures of full-length human NLRP3 in its native form and complexed with the inhibitor CRID3 (also named MCC950)(4). Inactive, ADP-bound NLRP3 is a decamer composed of homodimers of intertwined leucine-rich repeat (LRR) domains that assemble back-to-back as pentamers. The NACHT domain is located at the apical axis of this spherical structure. One pyrin domain dimer is in addition formed inside the LRR cage. Molecular contacts between the concave sites of two opposing LRR domains are mediated by an acidic loop that extends from an LRR transition segment. Binding of CRID3 considerably stabilizes the NACHT and LRR domains relative to each other. CRID3 binds into a cleft, connecting four subdomains of the NACHT with the transition LRR. Its central sulfonylurea group interacts with the Walker A motif of the NLRP3 nucleotide-binding domain and is sandwiched between two arginine residues, which explains the specificity of NLRP3 for this chemical entity. With the determination of the binding site of this key therapeutic agent, specific targeting of NLRP3 for the treatment of autoinflammatory and autoimmune diseases and rational drug optimization is within reach.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CRYSTAL-STRUCTURE; INFLAMMASOME; ACTIVATION; NEK7; REVEALS |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Feb 2024 06:57 |
| Last Modified: | 08 Feb 2024 06:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57388 |
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