Faber, Mirko and Wenzel, Juergen J. and Erl, Monika and Stark, Klaus and Schemmerer, Mathias (2022) No Evidence for Orthohepevirus C in Archived Human Samples in Germany, 2000-2020. VIRUSES-BASEL, 14 (4): 742. ISSN , 1999-4915
Full text not available from this repository. (Request a copy)Abstract
Orthohepevirus C1, also known as rat hepatitis E virus (HEV), has been shown to sporadically cause disease in immunocompromised and immunocompetent adults. While routine serological assays vary in reactivity, rat HEV is not detected in routine HEV RT-PCR. Thus, such infections could be either missed or misclassified as conventional HEV (Orthohepevirus A) infections. We conducted a retrospective screening study among serum and plasma samples from patients suspected of having HEV infection, which were archived at the national consultant laboratory for HAV and HEV between 2000 and 2020. We randomly selected n = 200 samples, which were initially tested reactive (positive or borderline) for HEV-IgM and negative for HEV RNA and re-examined them using a highly sensitive Orthohepevirus C genotype 1-specific in-house RT-qPCR (LoD 95: 6.73 copies per reaction) and a nested RT-PCR broadly reactive for Orthohepevirus A and C. Conventional sanger sequencing was conducted for resulting PCR products. No atypical HEV strains were detected (0 of 200 [0.0%; 95% confidence interval: 0.0%-1.89%], indicating that Orthohepevirus C infections in the investigated population (persons with clinical suspicion of hepatitis E and positive HEV-IgM) are very rare.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEPATITIS-E VIRUS; RAT; INFECTION; hepatitis E virus; orthohepevirus C; rat HEV; epidemiology; phylogeny; Germany |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Feb 2024 07:56 |
| Last Modified: | 20 Feb 2024 07:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57502 |
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