Yegorova, Alla and Skrypynets, Yuliia and Leonenko, Inna and Duerkop, Axel (2023) New terbium complex as a luminescent probe for determination of chlorogenic acid in green coffee and roasted coffee infusions. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 415 (2). pp. 235-244. ISSN 1618-2642, 1618-2650
Full text not available from this repository. (Request a copy)Abstract
Green coffee is coming into vogue as a food that contains remarkable contents of antioxidants like chlorogenic acid (ChA) and induces mild stimulation to the consumer. While most methods for determination of ChA require chromatographic separation prior its quantitation, we present the first probe and a simple, sensitive and validated luminescence method for the determination of chlorogenic acid in green and roasted coffee infusion samples that does not require a chromatographic separation. ChA can remarkably quench the luminescence intensity of the Tb3+ complex with 1-(furan-2-ylmethyl)-4-hydroxy-N-(4-methylpyridin-2-yl)-2-oxo-1,2,5,6,7,8-hexahydroquinoline-3-carboxamide (R-3) in aqueous solution containing urotropine buffer at a near neutral pH 7.5 at lambda(exc) = 315 nm and lambda(em) = 545 nm. Under optimal conditions, the quenching of the luminescence intensity is directly proportional to the concentration of ChA in the range of 0.5-30 mu g/mL, and the detection limit is 180 ng/mL. From measurements of luminescence decay time, it was determined that both static and dynamic quenching is induced upon coordination of ChA to Tb-R-3. The related quenching constants are K-S =5.97.10(4) M-1 and K-D =1.05.10(4) M-1. Finally, the method was applied successfully to the determination of ChA in real green and roasted coffee infusion samples and validated by HPLC to yield very closely matching concentrations of both methods. Therefore, this method can serve for a simple quality control of total ChA contents in coffee prior and after roasting.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | QUANTIFICATION; EXTRACTION; CAFFEINE; HPLC; Chlorogenic acid; Luminescence; Probe; Terbium complex; Quenching |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Feb 2024 05:59 |
| Last Modified: | 21 Feb 2024 05:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57528 |
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