da Costa, Kelly A. S. and Del Rosario, Joanne Marie M. and Ferrari, Matteo and Vishwanath, Sneha and Asbach, Benedikt and Kinsley, Rebecca and Wagner, Ralf and Heeney, Jonathan L. and Carnell, George W. and Temperton, Nigel J. (2022) Influenza A (N1-N9) and Influenza B (B/Victoria and B/Yamagata) Neuraminidase Pseudotypes as Tools for Pandemic Preparedness and Improved Influenza Vaccine Design. VACCINES, 10 (9): 1520. ISSN , 2076-393X
Full text not available from this repository. (Request a copy)Abstract
To better understand how inhibition of the influenza neuraminidase (NA) protein contributes to protection against influenza, we produced lentiviral vectors pseudotyped with an avian H11 hemagglutinin (HA) and the NA of all influenza A (N1-N9) subtypes and influenza B (B/Victoria and B/Yamagata). These NA viral pseudotypes (PV) possess stable NA activity and can be utilized as target antigens in in vitro assays to assess vaccine immunogenicity. Employing these NA PV, we developed an enzyme-linked lectin assay (pELLA) for routine serology to measure neuraminidase inhibition (NI) titers of reference antisera, monoclonal antibodies and post-vaccination sera with various influenza antigens. We also show that the pELLA is more sensitive than the commercially available NA-Fluor (TM) in detecting NA inhibition in these samples. Our studies may lead to establishing the protective NA titer that contributes to NA-based immunity. This will aid in the design of superior, longer lasting and more broadly protective vaccines that can be employed together with HA-targeted vaccines in a pre-pandemic approach.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VIRUS-LIKE PARTICLES; LINKED LECTIN; FLUOROMETRIC ASSAY; MOLECULAR-BASIS; GENE DELIVERY; HEMAGGLUTININ; ANTIBODY; H1N1; H5N1; TARGET; influenza; neuraminidase; pseudotype; ELLA; inhibition; vaccine; monoclonal antibody; antisera; immunity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Feb 2024 10:25 |
| Last Modified: | 20 Feb 2024 10:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57567 |
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