Lopez-Pajares, Vanessa and Qu, Kun and Zhang, Jiajing and Webster, Dan E. and Barajas, Brook C. and Siprashvili, Zurab and Zarnegar, Brian J. and Boxer, Lisa D. and Rios, Eon J. and Tao, Shiying and Kretz, Markus and Khavari, Paul A. (2015) A LncRNA-MAF:MAFB Transcription Factor Network Regulates Epidermal Differentiation. DEVELOPMENTAL CELL, 32 (6). pp. 693-706. ISSN 1534-5807, 1878-1551
Full text not available from this repository. (Request a copy)Abstract
Progenitor differentiation requires remodeling of genomic expression; however, in many tissues, such as epidermis, the spectrum of remodeled genes and the transcription factors (TFs) that control them are not fully defined. We performed kinetic transcriptome analysis during regeneration of differentiated epidermis and identified gene sets enriched in progenitors (594 genes), in early (159 genes), and in late differentiation (387 genes). Module mapping of 1,046 TFs identified MAF and MAFB as necessary and sufficient for progenitor differentiation. MAF: MAFB regulated 393 genes altered in this setting. Integrative analysis identified ANCR and TINCR lncRNAs as essential upstream MAF: MAFB regulators. ChIP-seq analysis demonstrated MAF: MAFB binding to known epidermal differentiation TF genes whose expression they controlled, including GRHL3, ZNF750, KLF4, and PRDM1. Each of these TFs rescued expression of specific MAF: MAFB target gene subsets in the setting of MAF: MAFB loss, indicating they act downstream of MAF: MAFB. A lncRNA-TF network is thus essential for epidermal differentiation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LONG NONCODING RNAS; PROMOTES OSTEOBLAST DIFFERENTIATION; TERMINAL DIFFERENTIATION; STEM-CELLS; HEMATOPOIETIC STEM; GENE-EXPRESSION; SOMATIC TISSUE; CHROMATIN; MAFB; COMMITMENT; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jul 2019 12:58 |
| Last Modified: | 23 Jul 2019 12:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5771 |
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