Shang, Renfu and Kretov, Dmitry A. and Adamson, Scott and Treiber, Thomas and Treiber, Nora and Vedanayagam, Jeffrey and Chuang, Jeffrey H. and Meister, Gunter and Cifuentes, Daniel and Lai, Eric C. (2022) Regulated dicing of pre-mir-144 via reshaping of its terminal loop. NUCLEIC ACIDS RESEARCH, 50 (13). pp. 7637-7654. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
Although the route to generate microRNAs (miRNAs) is often depicted as a linear series of sequential and constitutive cleavages, we now appreciate multiple alternative pathways as well as diverse strategies to modulate their processing and function. Here, we identify an unusually profound regulatory role of conserved loop sequences in vertebrate pre-mir-144, which are essential for its cleavage by the Dicer RNase III enzyme in human and zebrafish models. Our data indicate that pre-mir-144 dicing is positively regulated via its terminal loop, and involves the ILF3 complex (NF90 and its partner NF45/ILF2). We provide further evidence that this regulatory switch involves reshaping of the pre-mir-144 apical loop into a structure that is appropriate for Dicer cleavage. In light of our recent findings that mir-144 promotes the nuclear biogenesis of its neighbor mir-451, these data extend the complex hierarchy of nuclear and cytoplasmic regulatory events that can control the maturation of clustered miRNAs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-BINDING PROTEINS; MICRORNA BIOGENESIS; NUCLEAR FACTOR; CIRCULAR RNAS; NF90; LIN-28; NF45; EXPRESSION; PATHWAY; MIRNAS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Feb 2024 15:27 |
| Last Modified: | 22 Feb 2024 15:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57785 |
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