Cantoni, Diego and Mayora-Neto, Martin and Nadesalingam, Angalee and Wells, David A. and Carnell, George W. and Ohlendorf, Luis and Ferrari, Matteo and Palmer, Phil and Chan, Andrew C. Y. and Smith, Peter and Bentley, Emma M. and Einhauser, Sebastian and Wagner, Ralf and Page, Mark and Raddi, Gianmarco and Baxendale, Helen and Castillo-Olivares, Javier and Heeney, Jonathan and Temperton, Nigel (2022) Neutralisation Hierarchy of SARS-CoV-2 Variants of Concern Using Standardised, Quantitative Neutralisation Assays Reveals a Correlation With Disease Severity; Towards Deciphering Protective Antibody Thresholds. FRONTIERS IN IMMUNOLOGY, 13: 773982. ISSN 1664-3224,
Full text not available from this repository. (Request a copy)Abstract
The rise of SARS-CoV-2 variants has made the pursuit to define correlates of protection more troublesome, despite the availability of the World Health Organisation (WHO) International Standard for anti-SARS-CoV-2 Immunoglobulin sera, a key reagent used to standardise laboratory findings into an international unitage. Using pseudotyped virus, we examine the capacity of convalescent sera, from a well-defined cohort of healthcare workers (HCW) and Patients infected during the first wave from a national critical care centre in the UK to neutralise B.1.1.298, variants of interest (VOI) B.1.617.1 (Kappa), and four VOCs, B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma) and B.1.617.2 (Delta), including the B.1.617.2 K417N, informally known as Delta Plus. We utilised the WHO International Standard for anti-SARS-CoV-2 Immunoglobulin to report neutralisation antibody levels in International Units per mL. Our data demonstrate a significant reduction in the ability of first wave convalescent sera to neutralise the VOCs. Patients and HCWs with more severe COVID-19 were found to have higher antibody titres and to neutralise the VOCs more effectively than individuals with milder symptoms. Using an estimated threshold for 50% protection, 54 IU/mL, we found most asymptomatic and mild cases did not produce titres above this threshold.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SPIKE; CORONAVIRUS; COVID-19; variants of concern; correlates of protection (CoP); international standard; disease severity; 20; 136; IU; mL |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Feb 2024 15:53 |
| Last Modified: | 22 Feb 2024 15:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57805 |
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