Chavan, Sachin Ganpat and Yagati, Ajay Kumar and Koyappayil, Aneesh and Go, Anna and Yeon, Sangho and Lee, Min-Ho (2022) Recombinant Histidine-Tagged Nano-protein-based Highly Sensitive Electro-Sensing Device for Salivary Cortisol. BIOELECTROCHEMISTRY, 144: 108046. ISSN 1567-5394, 1878-562X
Full text not available from this repository. (Request a copy)Abstract
We have developed a powerful biosensing strategy for immobilizing histidine-tagged (His-Tag)-oriented recombinant nano-protein immobilization on a chemically modified glassy carbon electrode (GCE) surfaces via (S)-N-(5-amino-1-carboxypentyl)iminodiacetic acid (ANTA) acting as a chelating Ni2' centered interaction. Here, we introduce a label-free electro-sensor to quantify cortisol levels in saliva samples for point-of-care testing (POCT). The high specificity of the chemically modified GCE was established by genetically bio-engineered metal-binding sites on the selected recombinant apoferritin (R-AFTN) nano protein to impart functionality to its surface and by coating the carbon surface with the self assembled monolayers of 4-aminobenzoic acid (4-ABA) attached to ANTA groups complexed with Ni2' transition metal ions. Despite the variety of conventional assays available to monitor cortisol levels, they require bulky exterior outfits, which hinders use in the healthcare systems. Therefore, we performed a rapid, easy-to-implement, and low-cost quantitative electro-sensor to enable the real-time detection of cortisol levels in saliva samples. As a result, the cortisol electro-sensor fabricated with high specificity utilizing a GCE could measure cortisol levels with a detection limit of 0.95 ng/ml and sensitivity of 7.91 lA/ (ng/mL), which is a practical approach in human saliva. Thus, protein nanoprobe-based cortisol biosensing showed high sensitivity and selectivity for the direct electro-sensing of cortisol for POCT.(c) 2021 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CARBON ELECTRODE; NANOPARTICLES; APOFERRITIN; BIOSENSOR; IMMUNOASSAY; PLATFORM; BINDING; ASSAY; Electrophysiological cortisol sensor; Salivary stress testing; Glassy carbon electrode surface modification; (S)-N-(5-Amino-1-carboxypentyl)iminodiacetic acid (ANTA); R-AFTN nanoprobe; Antigen-antibody reaction |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jan 2024 13:34 |
| Last Modified: | 26 Jan 2024 13:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57828 |
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