Patrick, Joan and Alija, Mikel Garcia and Liebau, Jobst and Pettersson, Pontus and Metola, Ane and Maeler, Lena (2022) Dilute Bicelles for Glycosyltransferase Studies, Novel Bicelles with Phosphatidylinositol. JOURNAL OF PHYSICAL CHEMISTRY B, 126 (30). pp. 5655-5666. ISSN 1520-6106, 1520-5207
Full text not available from this repository. (Request a copy)Abstract
Solution-state NMR can be used to study protein- lipid interactions, in particular, the effect that proteins have on lipids. One drawback is that only small assemblies can be studied, and therefore, fast-tumbling bicelles are commonly used. Bicelles contain a lipid bilayer that is solubilized by detergents. A complication is that they are only stable at high concentrations, exceeding the CMC of the detergent. This issue has previously been addressed by introducing a detergent (Cyclosfos-6) with a substantially lower CMC. Here, we developed a set of bicelles using this detergent for studies of membrane-associated mycobacterial proteins, for example, PimA, a key enzyme for bacterial growth. To mimic the lipid composition of mycobacterial membranes, PI, PG, and PC lipids were used. Diffusion NMR was used to characterize the bicelles, and spin relaxation was used to measure the dynamic properties of the lipids. The results suggest that bicelles are formed, although they are smaller than "conventional " bicelles. Moreover, we studied the effect of MTSL-labeled PimA on bicelles containing PI and PC. The paramagnetic label was shown to have a shallow location in the bicelle, affecting the glycerol backbone of the lipids. We foresee that these bicelles will be useful for detailed studies of protein-lipid interactions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FAST-TUMBLING BICELLES; PHOSPHOLIPID-BILAYER NANODISCS; MAGNETIC-RESONANCE RELAXATION; MODEL-FREE APPROACH; MANNOSYLTRANSFERASE PIMA; MEMBRANE ASSOCIATION; DIFFUSION; BINDING; MACROMOLECULES; MORPHOLOGY |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Feb 2024 05:26 |
| Last Modified: | 16 Feb 2024 05:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57840 |
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