Miksys, Algirdas and Fu, Lifei and Madej, M. Gregor and Guerreiro, Duarte N. and Kaltwasser, Susann and Conway, Maria and Ejder, Sema and Bruckmann, Astrid and Marles-Wright, Jon and Lewis, Richard J. and O'Byrne, Conor and Pane-Farre, Jan and Ziegler, Christine (2022) Molecular insights into intra-complex signal transmission during stressosome activation. COMMUNICATIONS BIOLOGY, 5 (1): 621. ISSN 2399-3642
Full text not available from this repository. (Request a copy)Abstract
Cryo-EM structures of the stress-sensing complex in Listeria innocua reveal conformational changes that initiate the signaling response to environmental stress. The stressosome is a pseudo-icosahedral megadalton bacterial stress-sensing protein complex consisting of several copies of two STAS-domain proteins, RsbR and RsbS, and the kinase RsbT. Upon perception of environmental stress multiple copies of RsbT are released from the surface of the stressosome. Free RsbT activates downstream proteins to elicit a global cellular response, such as the activation of the general stress response in Gram-positive bacteria. The molecular events triggering RsbT release from the stressosome surface remain poorly understood. Here we present the map of Listeria innocua RsbR1/RsbS complex at resolutions of 3.45 angstrom for the STAS domain core in icosahedral symmetry and of 3.87 angstrom for the STAS domain and N-terminal sensors in D2 symmetry, respectively. The structure reveals a conformational change in the STAS domain linked to phosphorylation in RsbR. Docking studies indicate that allosteric RsbT binding to the conformationally flexible N-terminal sensor domain of RsbR affects the affinity of RsbS towards RsbT. Our results bring to focus the molecular events within the stressosome complex and further our understanding of this ubiquitous signaling hub.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-VIVO PHOSPHORYLATION; ENVIRONMENTAL-STRESS; BACILLUS-SUBTILIS; STRUCTURAL INSIGHTS; LIMITS ACTIVATION; SERINE KINASE; PATHWAY; PROTEIN; SIGMA(B); RSBR |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Christine Ziegler Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Feb 2024 05:54 |
| Last Modified: | 16 Feb 2024 05:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/57874 |
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