Arndt, Stephanie and Landthaler, Michael and Zimmermann, Julia L. and Unger, Petra and Wacker, Eva and Shimizu, Tetsuji and Li, Yang-Fang and Morfill, Gregor E. and Bosserhoff, Anja-Katrin and c, Sigrid (2015) Effects of Cold Atmospheric Plasma (CAP) on beta-Defensins, Inflammatory Cytokines, and Apoptosis-Related Molecules in Keratinocytes In Vitro and In Vivo. PLOS ONE, 10 (3): e0120041. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Cold atmospheric plasma (CAP) has been gaining increasing interest as a new approach for the treatment of skin diseases or wounds. Although this approach has demonstrated promising antibacterial activity, its exact mechanism of action remains unclear. This study explored in vitro and in vivo whether CAP influences gene expression and molecular mechanisms in keratinocytes. Our results revealed that a 2 min CAP treatment using the MicroPlaSter beta in analogy to the performed clinical studies for wound treatment induces expression of IL-8, TGF-beta 1, and TGF-beta 2. In vitro and in vivo assays indicated that keratinocyte proliferation, migration, and apoptotic mechanisms were not affected by the CAP treatment under the applied conditions. Further, we observed that antimicrobial peptides of the beta-defensin family are upregulated after CAP treatment. In summary, our results suggest that a 2 min application of CAP induces gene expression of key regulators important for inflammation and wound healing without causing proliferation, migration or cell death in keratinocytes. The induction of beta-defensins in keratinocytes describes an absolutely new plasma strategy. Activation of antimicrobial peptides supports the well-known antibacterial effect of CAP treatment, whereas the mechanism of beta-defensin activation by CAP is not investigated so far.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RANDOMIZED CONTROLLED-TRIAL; BARRIER DISCHARGE PLASMA; PRESSURE PLASMA; ARGON PLASMA; ANTIMICROBIAL PEPTIDES; HACAT-KERATINOCYTES; CHRONIC WOUNDS; HUMAN SKIN; CELLS; EXPRESSION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Jul 2019 13:08 |
| Last Modified: | 23 Jul 2019 13:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5791 |
Actions (login required)
 |
View Item |
