Huberdeau, Miguel Quevillon and Shah, Vivek Nilesh and Nahar, Smita and Neumeier, Julia and Houle, Francois and Bruckmann, Astrid and Gypas, Foivos and Nakanishi, Kotaro and Grosshans, Helge and Meister, Gunter and Simard, Martin J. (2022) A specific type of Argonaute phosphorylation regulates binding to microRNAs during C. elegans development. CELL REPORTS, 41 (11): 111822. ISSN 2211-1247
Full text not available from this repository. (Request a copy)Abstract
Argonaute proteins are at the core of the microRNA-mediated gene silencing pathway essential for animals. In C. elegans, the microRNA-specific Argonautes ALG-1 and ALG-2 regulate multiple processes required for proper animal developmental timing and viability. Here we identified a phosphorylation site on ALG-1 that modulates microRNA association. Mutating ALG-1 serine 642 into a phospho-mimicking residue impairs mi-croRNA binding and causes embryonic lethality and post-embryonic phenotypes that are consistent with alteration of microRNA functions. Monitoring microRNA levels in alg-1 phosphorylation mutant animals shows that microRNA passenger strands increase in abundance but are not preferentially loaded into ALG-1, indicating that the miRNA binding defects could lead to microRNA duplex accumulation. Our genetic and biochemical experiments support protein kinase A (PKA) KIN-1 as the putative kinase that phosphory-lates ALG-1 serine 642. Our data indicate that PKA triggers ALG-1 phosphorylation to regulate its microRNA association during C. elegans development.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEPENDENT PROTEIN-KINASE; CAENORHABDITIS-ELEGANS; SMALL-RNA; POSTTRANSCRIPTIONAL REGULATION; G-ALPHA(S) PATHWAY; CRYSTAL-STRUCTURE; STRUCTURAL BASIS; GENE; RISC; DEGRADATION |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Jan 2024 05:27 |
| Last Modified: | 30 Jan 2024 05:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58027 |
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