Makhneva, Ekaterina and Sklenarova, Dorota and Brandmeier, Julian C. and Hlavacek, Antonin and Gorris, Hans H. and Skladal, Petr and Farka, Zdenek (2022) Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays. ANALYTICAL CHEMISTRY, 94 (47). pp. 16376-16383. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels-horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)-UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROSTATE-SPECIFIC ANTIGEN; LINKED-IMMUNOSORBENT-ASSAY; UP-CONVERSION NANOPARTICLES; COLORIMETRIC IMMUNOASSAY; FLUORESCENCE IMMUNOASSAY; SENSITIVE DETECTION; MAGNETIC BEADS; CANCER; PSA; IMMUNOSENSOR |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Petra Gürster |
| Date Deposited: | 27 Jun 2024 10:29 |
| Last Modified: | 27 Jun 2024 10:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58036 |
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