Maurer, Wiebke and Hartmann, Nico and Argyriou, Loukas and Sossalla, Samuel and Streckfuss-Bömeke, Katrin (2022) Generation of homozygous Na(v)1.8 knock-out iPSC lines by CRISPR Cas9 genome editing to investigate a potential new antiarrhythmic strategy. STEM CELL RESEARCH, 60: 102677. ISSN 1873-5061, 1876-7753
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The sodium channel Na(v)1.8, encoded by SCN10A, is reported to contribute to arrhythmogenesis by inducing the late INa and thereby enhanced persistent Na+ current. However, its exact electrophysiological role in cardiomyocytes remains unclear. Here, we generated induced pluripotent stem cells (iPSCs) with a homozygous SCN10A knock-out from a healthy iPSC line by CRISPR Cas9 genome editing. The edited iPSCs maintained full pluripotency, genomic integrity, and spontaneous in vitro differentiation capacity. The iPSCs are able to differentiate into iPSC-cardiomyocytes, hence making it possible to investigate the role of Na(v)1.8 in the heart.
| Item Type: | Article |
|---|---|
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jan 2024 13:45 |
| Last Modified: | 26 Jan 2024 13:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58062 |
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