Zauhar, Randy and Biber, Josef and Jabri, Yassin and Kim, Mijin and Hu, Jian and Kaplan, Lew and Pfaller, Anna M. and Schaefer, Nicole and Enzmann, Volker and Schloetzer-Schrehardt, Ursula and Straub, Tobias and Hauck, Stefanie M. and Gamlin, Paul D. and McFerrin, Michael B. and Messinger, Jeffrey and Strang, Christianne E. and Curcio, Christine A. and Dana, Nicholas and Pauly, Diana and Grosche, Antje and Li, Mingyao and Stambolian, Dwight (2022) As in Real Estate, Location Matters: Cellular Expression of Complement Varies Between Macular and Peripheral Regions of the Retina and Supporting Tissues. FRONTIERS IN IMMUNOLOGY, 13: 895519. ISSN 1664-3224,
Full text not available from this repository. (Request a copy)Abstract
The cellular events that dictate the initiation of the complement pathway in ocular degeneration, such as age-related macular degeneration (AMD), is poorly understood. Using gene expression analysis (single cell and bulk), mass spectrometry, and immunohistochemistry, we dissected the role of multiple retinal and choroidal cell types in determining the complement homeostasis. Our scRNA-seq data show that the cellular response to early AMD is more robust in the choroid, particularly in fibroblasts, pericytes and endothelial cells. In late AMD, complement changes were more prominent in the retina especially with the expression of the classical pathway initiators. Notably, we found a spatial preference for these differences. Overall, this study provides insights into the heterogeneity of cellular responses for complement expression and the cooperation of neighboring cells to complete the pathway in healthy and AMD eyes. Further, our findings provide new cellular targets for therapies directed at complement.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PIGMENT EPITHELIUM; STRUCTURAL INSIGHT; DRUSEN FORMATION; KEY REGULATORS; MESSENGER-RNA; MOUSE MODEL; DEGENERATION; SYSTEM; ACTIVATION; CELLS; single cell; complement; age-related macular degeneration; retina; RPE; choroid |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Augenheilkunde Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Feb 2024 14:17 |
| Last Modified: | 27 Feb 2024 14:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58123 |
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