Bucchi, Cristina- and Ohlsson, Ella and de Anta, Josep Maria and Woelflick, Melanie and Galler, Kerstin and Manzanares-Cespedes, Maria Cristina and Widbiller, Matthias (2022) Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration? INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (5): 2830. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
The aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from human teeth. Both hAECs and hDPSCs were cultured with 10% FBS, eDMPs and an osteogenic differentiation medium (StemPro). Viability was assessed by MTT and cell adherence to dentin was evaluated by scanning electron microscopy. Furthermore, the expression of mineralization-, odontogenic differentiation- and epithelial-mesenchymal transition-associated genes was analyzed by quantitative real-time PCR, and mineralization was evaluated through Alizarin Red staining. The viability of hAECs was significantly lower compared with hDPSCs in all groups and at all time points. Both hAECs and hDPSCs adhered to dentin and were homogeneously distributed. The regulation of odontoblast differentiation- and mineralization-associated genes showed the lack of transition of hAECs into an odontoblastic phenotype; however, genes associated with epithelial-mesenchymal transition were significantly upregulated in hAECs. hAECs showed small amounts of calcium deposition after osteogenic differentiation with StemPro. Pluripotent hAECs adhere on dentin and possess the capacity to mineralize. However, they presented an unfavorable proliferation behavior and failed to undergo odontoblastic transition.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMMATURE PERMANENT TEETH; MESENCHYMAL STEM-CELLS; OSTEOGENIC DIFFERENTIATION; GENE-EXPRESSION; HUMAN TOOTH; TGF-BETA; DENTIN; ENDODONTICS; GROWTH; TRANSPLANTATION; human amnion epithelial cells; dental pulp stem cells; dentin matrix proteins; odontoblastic differentiation; revitalization |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Feb 2024 14:35 |
| Last Modified: | 29 Feb 2024 14:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58194 |
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