Kress, Sebastian and Schaller-Ammann, Roland and Feiel, Juergen and Wegener, Joachim and Priedl, Joachim and Dietrich, Wolf and Kasper, Cornelia and Egger, Dominik (2022) Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures. CELLS, 11 (3): 412. ISSN , 2073-4409
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The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well as cell lines were cultured on a collagen or gelatin methacryloyl (GelMA) hydrogel matrix, while monitoring relevant culture parameters and analytes. Comparing the interstitial fluid of the 3D models versus the corresponding culture medium, we found considerable differences in the concentrations of several analytes. These results clearly demonstrate that analyses of the culture medium only are not relevant for the development of standardized 3D culture processes. The presented bioreactor with an integrated sampling and sensor platform opens new horizons for the development, optimization, and standardization of 3D cultures. Furthermore, this technology holds the potential to reduce animal studies and improve the transferability of pharmaceutical in vitro studies by gaining more relevant results, bridging the gap towards clinical translation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; OPEN FLOW MICROPERFUSION; SANDWICH CULTURES; METABOLISM; GLUCOSE; PROLIFERATION; MECHANISMS; 3D culture; perfusion bioreactor; monitoring platform; sampling; analytics; cells; microenvironment; open flow microperfusion; sensors; 3R |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Feb 2024 14:51 |
| Last Modified: | 29 Feb 2024 14:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58258 |
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