Edfeldt, Gabriella and Lajoie, Julie and Rohl, Maria and Oyugi, Julius and Ahlberg, Alexandra and Khalilzadeh-Binicy, Behnaz and Bradley, Frideborg and Mack, Matthias and Kimani, Joshua and Omollo, Kenneth and Wahlby, Carolina and Fowke, Keith R. and Broliden, Kristina and Tjernlund, Annelie (2022) Regular Use of Depot Medroxyprogesterone Acetate Causes Thinning of the Superficial Lining and Apical Distribution of Human Immunodeficiency Virus Target Cells in the Human Ectocervix. JOURNAL OF INFECTIOUS DISEASES, 225 (7). pp. 1151-1161. ISSN 0022-1899, 1537-6613
Full text not available from this repository. (Request a copy)Abstract
Background The hormonal contraceptive depot medroxyprogesterone acetate (DMPA) may be associated with an increased risk of acquiring human immunodeficiency virus (HIV). We hypothesize that DMPA use influences the ectocervical tissue architecture and HIV target cell localization. Methods Quantitative image analysis workflows were developed to assess ectocervical tissue samples collected from DMPA users and control subjects not using hormonal contraception. Results Compared to controls, the DMPA group exhibited a significantly thinner apical ectocervical epithelial layer and a higher proportion of CD4(+)CCR5(+) cells with a more superficial location. This localization corresponded to an area with a nonintact E-cadherin net structure. CD4(+)Langerin(+) cells were also more superficially located in the DMPA group, although fewer in number compared to the controls. Natural plasma progesterone levels did not correlate with any of these parameters, whereas estradiol levels were positively correlated with E-cadherin expression and a more basal location for HIV target cells of the control group. Conclusions DMPA users have a less robust epithelial layer and a more apical distribution of HIV target cells in the human ectocervix, which could confer a higher risk of HIV infection. Our results highlight the importance of assessing intact genital tissue samples to gain insights into HIV susceptibility factors. By developing quantitative image analysis workflows, we revealed that women taking the hormonal contraceptive depot medroxyprogesterone acetate had a less robust ectocervical epithelium and a more superficial distribution of HIV target cells. This could confer a higher risk of HIV infection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN VAGINAL HISTOLOGY; HIV ACQUISITION; HORMONAL CONTRACEPTION; T-CELLS; POPULATIONS; THICKNESS; WOMEN; FLORA; female genital mucosa; hormonal contraception; DMPA; HIV; in situ staining; digital image analysis; epithelial integrity; HIV target cells; estradiol; progesterone |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nephrologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Feb 2024 07:18 |
| Last Modified: | 02 Feb 2024 07:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58269 |
Actions (login required)
 |
View Item |
