Pratesi, Federico and Errante, Fosca and Pacini, Lorenzo and Pena-Moreno, Irina Charlot and Quiceno, Sebastian and Carotenuto, Alfonso and Balam, Saidou and Konate, Drissa and Diakite, Mahamadou M. and Arevalo-Herrera, Myriam and Kajava, Andrey V. and Rovero, Paolo and Corradin, Giampietro and Migliorini, Paola and Papini, Anna M. and Herrera, Socrates (2022) A SARS-CoV-2 Spike Receptor Binding Motif Peptide Induces Anti-Spike Antibodies in Mice andIs Recognized by COVID-19 Patients. FRONTIERS IN IMMUNOLOGY, 13: 879946. ISSN 1664-3224,
Full text not available from this repository. (Request a copy)Abstract
The currently devastating pandemic of severe acute respiratory syndrome known as coronavirus disease 2019 or COVID-19 is caused by the coronavirus SARS-CoV-2. Both the virus and the disease have been extensively studied worldwide. A trimeric spike (S) protein expressed on the virus outer bilayer leaflet has been identified as a ligand that allows the virus to penetrate human host cells and cause infection. Its receptor-binding domain (RBD) interacts with the angiotensin-converting enzyme 2 (ACE2), the host-cell viral receptor, and is, therefore, the subject of intense research for the development of virus control means, particularly vaccines. In this work, we search for smaller fragments of the S protein able to elicit virus-neutralizing antibodies, suitable for production by peptide synthesis technology. Based on the analysis of available data, we selected a 72 aa long receptor binding motif (RBM436-507) of RBD. We used ELISA to study the antibody response to each of the three antigens (S protein, its RBD domain and the RBM436-507 synthetic peptide) in humans exposed to the infection and in immunized mice. The seroreactivity analysis showed that anti-RBM antibodies are produced in COVID-19 patients and immunized mice and may exert neutralizing function, although with a frequency lower than anti-S and -RBD. These results provide a basis for further studies towards the development of vaccines or treatments focused on specific regions of the S virus protein, which can benefit from the absence of folding problems, conformational constraints and other advantages of the peptide synthesis production.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN SECONDARY STRUCTURE; VACCINE; SARS-CoV-2; receptor binding motif; COVID-19; immunized animals; neutralizing Abs; spike (S) protein |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nephrologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2024 14:26 |
| Last Modified: | 01 Feb 2024 14:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58348 |
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