Demais, Valerie and Pohl, Anne and Wunderlich, Kirsten A. and Pfaller, Anna M. and Kaplan, Lew and Barthelemy, Amelie and Dittrich, Robin and Puig, Berta and Giebel, Bernd and Hauck, Stefanie M. and Pfrieger, Frank W. and Grosche, Antje (2022) Release of VAMP5-positive extracellular vesicles by retinal Muller glia in vivo. JOURNAL OF EXTRACELLULAR VESICLES, 11 (9): e12254. ISSN , 2001-3078
Full text not available from this repository. (Request a copy)Abstract
Cell-cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The specific mechanisms employed by each cell type in the brain are incompletely understood. Here, we explored the means of communication used by Muller cells, a type of radial glial cells in the retina, which forms part of the central nervous system. Using immunohistochemical, electron microscopic, and molecular analyses, we provide evidence for the release of distinct extracellular vesicles from endfeet and microvilli of retinal Muller cells in adult mice in vivo. We identify VAMP5 as a Muller cell-specific SNARE component that is part of extracellular vesicles and responsive to ischemia, and we reveal differences between the secretomes of immunoaffinity-purified Muller cells and neurons in vitro. Our findings suggest extracellular vesicle-based communication as an important mediator of cellular interactions in the retina.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CARBONIC-ANHYDRASE-XIV; PIGMENT EPITHELIUM; PROTEOMIC ANALYSIS; AQUEOUS-HUMOR; EXOSOMES; CELLS; ASTROCYTES; EXPRESSION; SECRETION; PROTEINS; neuroglia; exosomes; tetraspanin; gliosis; SNARE complex; secretome; vitreous body; microvillus |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Humangenetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2024 14:22 |
| Last Modified: | 01 Feb 2024 14:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58363 |
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