Li, Ling and Hou, Saiying and Xiang, Wei and Song, Zihan and Wang, Yuan and Zhang, Li and Li, Jing and Gu, Hongya and Dong, Juan and Dresselhaus, Thomas and Zhong, Sheng and Qu, Li-Jia (2022) The egg cell is preferentially fertilized in Arabidopsis double fertilization. JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 64 (11). pp. 2039-2046. ISSN 1672-9072, 1744-7909
Full text not available from this repository. (Request a copy)Abstract
In flowering plants (angiosperms), fertilization of the egg cell by one sperm cell produces an embryo, whereas fusion of a second sperm cell with the central cell generates the endosperm. In most angiosperms like Arabidopsis, a pollen grain contains two isomorphic sperm cells required for this double fertilization process. A long-standing unsolved question is whether the two fertilization events have any preference. A tool to address this question is the usage of the cyclin-dependent kinase a1 (cdka;1) mutant pollen, which produces a single sperm-like cell (SLC). Here, we first adopt a complementation-based fluorescence-labeling method to successfully separate and collect cdka;1 mutant pollen containing a single SLC. Single-cell RNA-sequencing analysis revealed that cdka;1 SLCs show a gene expression profile highly similar to that of sperm cells and not to the generative cell, precursor of the two sperm cells. Pollination assays using a limited number of cdka;1 mutant pollen revealed that in 98.2% of the ovules, single fertilization of the egg cell occurred. Pollinartion of pistils with excessive cdka;1 mutant pollen allowed the delivery of a second SLC via fertilization recovery, which fertilized the central cell, resulting in 20.7% double-fertilized ovules. This indicates that cdka;1 SLCs are able to fertilize both the egg and the central cell. Taken together, our findings have answered a long-standing question and support that preferential fertilization of the egg cell is evident in Arabidopsis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOSPERM DEVELOPMENT; GAMETE FUSION; RNA-SEQ; POLLEN; PROLIFERATION; MUTANT; BLOCK; TRANSCRIPTOMICS; CYCLE; FATE; Arabidopsis; double fertilization; egg cell; preferential fertilization |
| Subjects: | 500 Science > 550 Earth sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2024 13:58 |
| Last Modified: | 01 Feb 2024 13:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58368 |
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