Cai, Hanyang and Liu, Liping and Huang, Youmei and Zhu, Wenhui and Qi, Jingang and Xi, Xinpeng and Aslam, Mohammad and Dresselhaus, Thomas and Qin, Yuan (2022) Brassinosteroid signaling regulates female germline specification in Arabidopsis. CURRENT BIOLOGY, 32 (5). 1102-1114.e5. ISSN 0960-9822, 1879-0445
Full text not available from this repository. (Request a copy)Abstract
Unlike in humans and animals, plant germlines are specified de novo from somatic cells in the reproductive organs of the flower. In most flowering plant ovules, the female germline starts with the differentiation of one megaspore mother cell (MMC), which initiates a developmental program distinct from adjoining cells. Phytohormones act as a key player in physiological processes during plant development, in particular by providing positional information that supports localized differentiation events. However, little is known about the role of phytohormones for female germline initiation and establishment. Using Arabidopsis as a flowering plant model, we show that brassinosteroid (BR) biosynthesis and signaling components are accumulated in sporophytic cells of ovule primordia but not in the megaspore mother cell representing the precursor of the female germline. We further demonstrate that BR signaling restricts multiple sub-epidermal cells in the distal nucellus region of ovule primordia from acquiring MMC-like cell identity by transiently activating the WRKY23 transcription factor, expressed exclusively in L2 layer cells adjacent to the MMC. This activation is regulated through the BRI1 receptor and directly by the BZR1 transcriptional repressor family. Mutations in BR biosynthesis or signaling components and ectopic activation of BR signaling in MMCs induce multiple MMC-like cells. In summary, our findings elucidate a gene regulatory network that shows how the hormone BR generated in sporophytic ovule primordia cells restricts the origin of the female germline to a single cell.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OVULE DEVELOPMENT; WILD-TYPE; ROOT DEVELOPMENT; GENE-EXPRESSION; AUXIN; MEIOSIS; BIOSYNTHESIS; ACTIVATION; GROWTH; DIFFERENTIATION |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Jan 2024 05:40 |
| Last Modified: | 30 Jan 2024 05:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58410 |
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