Banks, William A. and Sharma, Priyanka and Hansen, Kim M. and Ludwig, Nils and Whiteside, Theresa L. (2022) Characteristics of Exosomes and the Vascular Landscape Regulate Exosome Sequestration by Peripheral Tissues and Brain. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (20): 12513. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Exosomes mediate intercellular communication, shuttling messages between cells and tissues. We explored whether exosome tissue sequestration is determined by the exosomes or the tissues using ten radiolabeled exosomes from human or murine, cancerous or noncancerous cell lines. We measured sequestration of these exosomes by the liver, kidney, spleen, and lung after intravenous injection into male CD-1 mice. Except for kidney sequestration of three exosomes, all exosomes were incorporated by all tissues, but sequestration levels varied greatly among exosomes and tissues. Species of origin (mouse vs. human) or source (cancerous vs. noncancerous cells) did not influence tissue sequestration. Sequestration of J774A.1 exosomes by liver involved the mannose-6 phosphate (M6P) receptor. Wheatgerm agglutinin (WGA) or lipopolysaccharide (LPS) treatments enhanced sequestration of exosomes by brain and lung but inhibited sequestration by liver and spleen. Response to LPS was not predictive of response to WGA. Path and heat map analyses included our published results for brain and found distinct clusters among the exosomes and the tissues. In conclusion, we found no evidence for a universal binding site controlling exosome-tissue interactions. Instead, sequestration of exosomes by tissues is differentially regulated by both exosomes and tissues and may be stimulated or inhibited by WGA and inflammation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXTRACELLULAR VESICLES; CELL-SURFACE; exosomes; liver; kidney; brain; spleen; lung; lipopolysaccharide; inflammation; wheatgerm agglutinin; mannose-6 phosphate receptor |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2024 09:36 |
| Last Modified: | 01 Feb 2024 09:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58444 |
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