Barone, Monica and Garelli, Silvia and Rampelli, Simone and Agostini, Alessandro and Matysik, Silke and D'Amico, Federica and Krautbauer, Sabrina and Mazza, Roberta and Salituro, Nicola and Fanelli, Flaminia and Iozzo, Patricia and Sanz, Yolanda and Candela, Marco and Brigidi, Patrizia and Pagotto, Uberto and Turroni, Silvia (2022) Multi-omics gut microbiome signatures in obese women: role of diet and uncontrolled eating behavior. BMC MEDICINE, 20 (1): 500. ISSN 1741-7015,
Full text not available from this repository. (Request a copy)Abstract
Background: Obesity and related co-morbidities represent a major health challenge nowadays, with a rapidly increasing incidence worldwide. The gut microbiome has recently emerged as a key modifier of human health that can affect the development and progression of obesity, largely due to its involvement in the regulation of food intake and metabolism. However, there are still few studies that have in-depth explored the functionality of the human gut microbiome in obesity and even fewer that have examined its relationship to eating behaviors. Methods: In an attempt to advance our knowledge of the gut-microbiome-brain axis in the obese phenotype, we thoroughly characterized the gut microbiome signatures of obesity in a well-phenotyped Italian female cohort from the NeuroFAST and MyNewGut EU FP7 projects. Fecal samples were collected from 63 overweight/obese and 37 normal-weight women and analyzed via a multi-omics approach combining 16S rRNA amplicon sequencing, metagenomics, metatranscriptomics, and lipidomics. Associations with anthropometric, clinical, biochemical, and nutritional data were then sought, with particular attention to cognitive and behavioral domains of eating. Results: We identified four compositional clusters of the gut microbiome in our cohort that, although not distinctly associated with weight status, correlated differently with eating habits and behaviors. These clusters also differed in functional features, i.e., transcriptional activity and fecal metabolites. In particular, obese women with uncontrolled eating behavior were mostly characterized by low-diversity microbial steady states, with few and poorly interconnected species (e.g., Ruminococcus torques and Bifidobacterium spp.), which exhibited low transcriptional activity, especially of genes involved in secondary bile acid biosynthesis and neuroendocrine signaling (i.e., production of neurotransmitters, indoles and ligands for cannabinoid receptors). Consistently, high amounts of primary bile acids as well as sterols were found in their feces. Conclusions: By finding peculiar gut microbiome profiles associated with eating patterns, we laid the foundation for elucidating gut-brain axis communication in the obese phenotype. Subject to confirmation of the hypotheses herein generated, our work could help guide the design of microbiome-based precision interventions, aimed at rewiring microbial networks to support a healthy diet-microbiome-gut-brain axis, thus counteracting obesity and related complications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FOOD ADDICTION; INSULIN; GLUCOSE; HOMEOSTASIS; REWARD; MUCIN; GABA; Gut microbiome; Obesity; Diet; Uncontrolled eating behavior; Metagenomics; Metatranscriptomics; Lipidomics; Gut-brain axis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2024 07:43 |
| Last Modified: | 01 Feb 2024 07:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58492 |
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