Sohl, Julian and Hartmann, Ann-Kathrin and Hahlbrock, Jennifer and Bartneck, Joschka and Stassen, Michael and Klein, Matthias and Bros, Matthias and Grabbe, Stephan and Marini, Federico and Woods, Kevin and Guezguez, Borhane and Mack, Matthias and Schild, Hansjorg and Muth, Sabine and Melchior, Felix and Probst, Hans Christian and Langguth, Peter and Radsak, Markus P. (2022) Dithranol as novel co-adjuvant for non-invasive dermal vaccination. NPJ VACCINES, 7 (1): 112. ISSN , 2059-0105
Full text not available from this repository. (Request a copy)Abstract
Transcutaneous immunization (TCI) utilizing the TLR7 agonist imiquimod (IMQ-TCI) induces T cell-driven protective immunity upon application onto intact skin. In our present work, we combine the anti-psoriatic agent dithranol with IMQ-TCI to boost vaccination efficacy (Dithranol/IMQ-based transcutaneous vaccination (DIVA)). Using ovalbumin-derived peptides as model antigens in mice, DIVA induced superior cytolytic CD8(+) T cells and CD4(+) T cells with a T-H1 cytokine profile in the priming as well as in the memory phase. Regarding the underlying mechanisms, dithranol induced an oxidant-dependent, monocyte-attracting inflammatory milieu in the skin boosting TLR7-dependent activation of dendritic cells and macrophages leading to superior T cell priming and protective immunity in vaccinia virus infection. In conclusion, we introduce the non-invasive vaccination method DIVA to induce strong primary and memory T cell responses upon a single local treatment. This work provides relevant insights in cutaneous vaccination approaches, paving the way for clinical development in humans.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSCUTANEOUS IMMUNIZATION; CUTTING EDGE; EXPRESSION; PSORIASIS; RESPONSES; IMMUNITY; CELLS; CTL; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nephrologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2024 07:04 |
| Last Modified: | 01 Feb 2024 07:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58549 |
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