Krause, Susanne and Gfrerer, Sabrina and von Kuegelgen, Andriko and Reuse, Carsten and Dombrowski, Nina and Villanueva, Laura and Bunk, Boyke and Sproeer, Cathrin and Neu, Thomas R. and Kuhlicke, Ute and Schmidt-Hohagen, Kerstin and Hiller, Karsten and Bharat, Tanmay A. M. and Rachel, Reinhard and Spang, Anja and Gescher, Johannes (2022) The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host. NATURE COMMUNICATIONS, 13 (1): 1735. ISSN , 2041-1723
Full text not available from this repository. (Request a copy)Abstract
Micrarchaeota is a distinctive lineage assigned to the DPANN archaea, which includes poorly characterised microorganisms with reduced genomes that likely depend on interactions with hosts for growth and survival. Here, we report the enrichment of a stable co-culture of a member of the Micrarchaeota (Ca. Micrarchaeum harzensis) together with its Thermoplasmatales host (Ca. Scheffleriplasma hospitalis), as well as the isolation of the latter. We show that symbiont-host interactions depend on biofilm formation as evidenced by growth experiments, comparative transcriptomic analyses and electron microscopy. In addition, genomic, metabolomic, extracellular polymeric substances and lipid content analyses indicate that the Micrarchaeon symbiont relies on the acquisition of metabolites from its host. Our study of the cell biology and physiology of a Micrarchaeon and its host adds to our limited knowledge of archaeal symbioses. The Micrarchaeota lineage includes poorly characterized archaea with reduced genomes that likely depend on host interactions for survival. Here, the authors report a stable co-culture of a member of the Micrarchaeota and its host, and use multi-omic and physiological analyses to shed light on this symbiosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-SITU DETECTION; NANOARCHAEUM-EQUITANS; ARCHAEA; ALIGNMENT; INSIGHTS; GENOME; TREE; PERFORMANCE; DIVERSITY; EVOLUTION; |
| Subjects: | 500 Science > 550 Earth sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2024 07:06 |
| Last Modified: | 01 Feb 2024 07:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58552 |
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