Lenti, Elisa and Genovese, Luca and Bianchessi, Silvia and Maurizio, Aurora and Sain, Simona Baghai and di Lillo, Alessia and Mattavelli, Greta and Harel, Itamar and Bernassola, Francesca and Hehlgans, Thomas and Pfeffer, Klaus and Crosti, Mariacristina and Abrignani, Sergio and Evans, Sylvia M. and Sitia, Giovanni and Guimaraes-Camboa, Nuno and Russo, Vincenzo and van de Pavert, Serge A. and Garcia-Manteiga, Jose Manuel and Brendolan, Andrea (2022) Fate mapping and scRNA sequencing reveal origin and diversity of lymph node stromal precursors. IMMUNITY, 55 (4). 606-622.e6. ISSN 1074-7613, 1097-4180
Full text not available from this repository. (Request a copy)Abstract
Lymph node (LN) stromal cells play a crucial role in LN development and in supporting adaptive immune responses. However, their origin, differentiation pathways, and transcriptional programs are still elusive. Here, we used lineage-tracing approaches and single-cell transcriptome analyses to determine origin, transcriptional profile, and composition of LN stromal and endothelial progenitors. Our results showed that all major stromal cell subsets and a large proportion of blood endothelial cells originate from embryonic Hoxb6(+) progenitors of the lateral plate mesoderm (LPM), whereas lymphatic endothelial cells arise from Pax3(+) progenitors of the paraxial mesoderm (PXM). Single-cell RNA sequencing revealed the existence of different Cd34(+) and Cxcl13(+) stromal cell subsets and showed that embryonic LNs contain proliferating progenitors possibly representing the amplifying populations for terminally differentiated cells. Taken together, our work identifies the earliest embryonic sources of LN stromal and endothelial cells and demonstrates that stromal diversity begins already during LN development.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FIBROBLASTIC RETICULAR CELLS; NEURAL CREST; ENDOTHELIAL-CELLS; ORGANIZER; NETWORKS; ORGANOGENESIS; ACTIVATION; DYNAMICS; PATHWAY; ATLAS |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Jan 2024 05:48 |
| Last Modified: | 30 Jan 2024 05:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58580 |
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