Schoenberger, Stefan and Mohseni, Mahsa Mir and Ellinger, Joerg and Tran, Giao Vu Quynh and Becker, Martina and Claviez, Alexander and Classen, Carl-Friedrich and Hermes, Barbara and Driever, Pablo Hernaiz and Jorch, Norbert and Lauten, Melchior and Mehlitz, Marcus and Schaefer, Niklas and Scheer-Preiss, Johanna and Schneider, Dominik T. and Troeger, Anja and Calaminus, Gabriele and Dilloo, Dagmar (2023) MicroRNA-profiling of miR-371 similar to 373-and miR-302/367-clusters in serum and cerebrospinal fluid identify patients with intracranial germ cell tumors. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 149 (2). pp. 791-802. ISSN 0171-5216, 1432-1335
Full text not available from this repository. (Request a copy)Abstract
Purpose Intracranial germ cell tumors (iGCT) comprise germinoma and non-germinoma. Their diagnosis predominantly relies on biopsy as only one-fifth of patients present with elevated biomarkers (AFP/beta-HCG) in serum or cerebrospinal fluid (CSF). MicroRNAs (miR/miRNA) have emerged as non-invasive biomarkers in extracranial GCT and may potentially facilitate non-invasive diagnosis in iGCT. Methods We analyzed eight miRNAs in serum and CSF from the miR-371 similar to 373- and miR-302/367-clusters and four miRNAs differentially expressed in iGCT tissue (miR-142-5p/miR-146a-5p/miR-335-5p/miR-654-3p) from eight iGCT patients (age 10-33 years) and 12 control subjects by pre-amplified RT-qPCR. MiR-30b-5p (serum) and miR-204-5p (CSF) acted as reference genes. Delta C-t-values were expressed as 2(-Delta)(Delta Ct) after standardization against controls. Results Between iGCT and control patients' serum C t -values of miR-371a-3p (p = 0.0159), miR-372-3p (p= 0.0095, miR367 (p = 0.0190), miR-302a (p = 0.0381) and miR-302d-3p (p = 0.0159) differed significantly. Discriminatory pattern in CSF was similar to serum as miR-371a (p = 0.0286), miR-372-3p (p = 0.0028), miR-367-3p (p = 0.0167) and miR-302d-3p (p = 0.0061) distinguished between patients and controls. Abundant 2(-Delta)(Delta C)(t) levels of each of these miRNAs were found across all serum and CSF samples including biomarker-negative patients. Conclusion With the largest data set so far, we underline the suitability of miR-371a, miR-372, miR-367 and miR-302d in serum and CSF for diagnosis of iGCT, particularly in biomarker-negative germinoma. Diagnosis of iGCT by miRNA analysis is a feasible and valid approach, particularly as serum can be readily obtained by a less invasive procedure. MiRNA analysis may discriminate iGCT from other tumors with similar radiological findings and may allow to monitor response to therapy as well as early relapse during follow-up.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CENTRAL-NERVOUS-SYSTEM; EXPRESSION; CNS; DIAGNOSIS; BIOMARKER; Intracranial germ cell tumors; Liquid biopsy; miR-371; miR-372; miR-367; miR-302d |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Pädiatrische Hämatologie, Onkologie und Stammzelltransplantation |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Mar 2024 13:54 |
| Last Modified: | 05 Mar 2024 13:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58668 |
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