Hammer, Nadine and Brandl, Ferdinand P. and Kirchhof, Susanne and Messmann, Viktoria and Goepferich, Achim M. (2015) Protein Compatibility of Selected Cross-linking Reactions for Hydrogels. MACROMOLECULAR BIOSCIENCE, 15 (3). pp. 405-413. ISSN 1616-5187, 1616-5195
Full text not available from this repository. (Request a copy)Abstract
The compatibility of selected cross-linking reactions with lysozyme is investigated. Michael-type additions of nucleophilic amino acids to maleimide, vinyl sulfone and acrylamide groups are detected by gel electrophoresis. The degree of modification depends on the polymer and the pH. Complete modification with more than five PEG chains is observed after incubation with mPEG5k-vinyl sulfone at pH 9, whereas 96% of the protein remains unmodified after incubation with mPEG5k-acrylamide at pH 4. Incubation with mPEG5k-thiol results in thiol-disulfide exchange reactions. Hydrogel preparation is simulated by using polymer mixtures. Protein modifications are detected, which may affect the protein structure, decrease activity and bioavailability, and increase the risk for immune responses.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EGG-WHITE LYSOZYME; DRUG-DELIVERY APPLICATIONS; CONJUGATE ADDITION; TRIGGERED RELEASE; DEXTRAN HYDROGELS; GROWTH-FACTOR; IMMOBILIZATION; DESIGN; PEGYLATION; CHALLENGES; compatibility; crosslinking; drug delivery systems; hydrogels; proteins |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Jul 2019 11:15 |
| Last Modified: | 24 Jul 2019 11:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5870 |
Actions (login required)
 |
View Item |
