Seeholzer, Theresa and Tarau, Daniela and Hollendonner, Lea and Auer, Andrea and Rachel, Reinhard and Grohmann, Dina and Giessibl, Franz J. and Weymouth, Alfred J. (2023) A Next-Generation qPlus-Sensor-Based AFM Setup: Resolving Archaeal S-Layer Protein Structures in Air and Liquid. JOURNAL OF PHYSICAL CHEMISTRY B, 127 (31). pp. 6949-6957. ISSN 1520-6106, 1520-5207
Full text not available from this repository. (Request a copy)Abstract
Surface-layer (S-layer) proteins form the outermost envelopeinmany bacteria and most archaea and arrange in two-dimensional quasicrystallinestructures via self-assembly. We investigated S-layer proteins extractedfrom the archaeon Pyrobaculum aerophilium with aqPlus sensor-based atomic force microscope (AFM) in both liquid andambient conditions and compared it to transmission electron microscopy(TEM) images under vacuum conditions. For AFM scanning, a next-generationliquid cell and a new protocol for creating long and sharp sapphiretips was introduced. Initial AFM images showed only layers of residualdetergent molecules (sodium dodecyl sulfate, SDS), which are usedto isolate the S-layer proteins from the cells. SDS was not visiblein the TEM images, requiring more thorough sample preparation forAFM measurements. These improvements allowed us to resolve the crystallikestructure of the S-layer samples with frequency-modulation AFM inboth air and liquid.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ATOMIC-FORCE MICROSCOPY; RESOLUTION; DEPOSITION; SURFACES; LATTICES; KIT |
| Subjects: | 500 Science > 530 Physics 500 Science > 570 Life sciences |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Chair Professor Giessibl > Group Franz J. Giessibl Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Dina Grohmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Feb 2024 07:48 |
| Last Modified: | 21 Feb 2024 07:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58881 |
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