Bludau, Anna and Schwartz, Uwe and Zeitler, Daniela M. and Royer, Melanie and Meister, Gunter and Neumann, Inga D. and Menon, Rohit (2023) Functional involvement of septal miR-132 in extinction and oxytocin-mediated reversal of social fear. MOLECULAR PSYCHIATRY. ISSN 1359-4184, 1476-5578
Full text not available from this repository. (Request a copy)Abstract
Social interactions are critical for mammalian survival and evolution. Dysregulation of social behavior often leads to psychopathologies such as social anxiety disorder, denoted by intense fear and avoidance of social situations. Using the social fear conditioning (SFC) paradigm, we analyzed expression levels of miR-132-3p and miR-124-3p within the septum, a brain region essential for social preference and avoidance behavior, after acquisition and extinction of social fear. Here, we found that SFC dynamically altered both microRNAs. Functional in vivo approaches using pharmacological strategies, inhibition of miR-132-3p, viral overexpression of miR-132-3p, and shRNA-mediated knockdown of miR-132-3p specifically within oxytocin receptor-positive neurons confirmed septal miR-132-3p to be critically involved not only in social fear extinction, but also in oxytocin-induced reversal of social fear. Moreover, Argonaute-RNA-co-immunoprecipitation-microarray analysis and further in vitro and in vivo quantification of target mRNA and protein, revealed growth differentiation factor-5 (Gdf-5) as a target of miR-132-3p. Septal application of GDF-5 impaired social fear extinction suggesting its functional involvement in the reversal of social fear. In summary, we show that septal miR-132-3p and its downstream target Gdf-5 regulate social fear expression and potentially mediate oxytocin-induced reversal of social fear.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMPAIRS MEMORY; EXPRESSION; ANXIETY; MICRORNA-132; DEGRADATION; RECEPTOR; NEURONS |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Tierphysiologie/Neurobiologie (Prof. Dr. Inga Neumann) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Feb 2024 08:41 |
| Last Modified: | 21 Feb 2024 08:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58886 |
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