Pienimaeki-Roemer, Annika and Kuhlmann, Katja and Boettcher, Alfred and Konovalova, Tatiana and Black, Anne and Orso, Evelyn and Liebisch, Gerhard and Ahrens, Maike and Eisenacher, Martin and Meyer, Helmut E. and Schmitz, Gerd (2015) Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets. TRANSFUSION, 55 (3). pp. 507-521. ISSN 0041-1132, 1537-2995
Full text not available from this repository. (Request a copy)Abstract
BackgroundPlatelets (PLTs) in stored PLT concentrates (PLCs) release PLT extracellular vesicles (PL-EVs) induced by senescence and activation, resembling the PLT storage lesion. No comprehensive classification or molecular characterization of senescence-induced PL-EVs exists to understand PL-EV heterogeneity. Study Design and MethodsPL-EVs from 5-day-stored PLCs from healthy individuals were isolated and subfractionated by differential centrifugation, filtration, and density gradient ultracentrifugation into five PLT microvesicle (PL-MV) subfractions (Fraction [F]1-F5) and PLT exosomes (PL-EXs). PL-EV size, concentration, and composition were analyzed by nanoparticle tracking analysis, flow cytometry, and lipid and protein mass spectrometry. Protein data were verified by Western blot. ResultsPL-EVs showed overlapping mean particle sizes of 180 to 260nm, but differed significantly in composition. Less dense, intermediate, and dense PL-MVs enriched specific lipidomic and proteomic markers related to the plasma membrane, intracellular membranes, PLT granules, mitochondria, and PLT activation. -Synuclein (81% of total) accumulated in F1 and F2, amyloid- (A) precursor protein in F3 and F4 (84%), and apolipoprotein (Apo)E (88%) and ApoJ (92%) in F3 to F5. PL-EXs enriched lipid species and proteins, with high abundance of lipid raft, PLT adhesion, and immune response-related markers. ConclusionDifferential lipid and protein compositions of PL-EVs suggest their unique cellular origins and functions, partly overlapping with PLT granule secretion. Dense PL-MVs might represent autophagic vesicles released during PLT activation and apoptosis and PL-EXs resemble lipid rafts, with a potential role in PLT aggregation and immunity. Segregation of -synuclein and A precursor protein, ApoE, and ApoJ into less dense and dense PL-MVs, respectively, show their differential carrier role of neurologic disease-related cargo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; NANOPARTICLE TRACKING ANALYSIS; CELL-DERIVED MICROPARTICLES; ALZHEIMERS AMYLOID-BETA; ALPHA-SYNUCLEIN; HUMAN PLASMA; AMINOPHOSPHOLIPID TRANSLOCASE; PARKINSONS-DISEASE; VASCULAR PATHOLOGY; BRAIN MICROBLEEDS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jul 2019 05:49 |
| Last Modified: | 26 Jul 2019 05:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5890 |
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