Stoeckl, Richard and Nissl, Laura and Reichelt, Robert and Rachel, Reinhard and Grohmann, Dina and Gruenberger, Felix (2023) The transcriptional regulator EarA and intergenic terminator sequences modulate archaellation in Pyrococcus furiosus. FRONTIERS IN MICROBIOLOGY, 14: 1241399. ISSN 1664-302X
Full text not available from this repository. (Request a copy)Abstract
The regulation of archaellation, the formation of archaeal-specific cell appendages called archaella, is crucial for the motility, adhesion, and survival of archaeal organisms. Although the heavily archaellated and highly motile Pyrococcus furiosus is a key model organism for understanding the production and function of archaella in Euryarchaea, the transcriptional regulation of archaellum assembly is so far unknown. Here we show that the transcription factor EarA is the master regulator of the archaellum (arl) operon transcription, which is further modulated by intergenic transcription termination signals. EarA deletion or overexpression strains demonstrate that EarA is essential for archaellation in P. furiosus and governs the degree of archaellation. Providing a single-molecule update on the transcriptional landscape of the arl operon in P. furiosus, we identify sequence motifs for EarA binding upstream of the arl operon and intergenic terminator sequences as critical elements for fine-tuning the expression of the multicistronic arl cluster. Furthermore, transcriptome re-analysis across different Thermococcales species demonstrated a heterogeneous production of major archaellins, suggesting a more diverse composition of archaella than previously recognized. Overall, our study provides novel insights into the transcriptional regulation of archaellation and highlights the essential role of EarA in Pyrococcus furiosus. These findings advance our understanding of the mechanisms governing archaellation and have implications for the functional diversity of archaella.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARCHAEAL TRANSCRIPTION; HYDROTHERMAL VENT; SP. NOV.; FLAGELLA; GENES; ACTIVATOR; PROMOTER; OPERON; MUTANT; Archaea; archaellum; transcriptomics; EarA; Thermococcales; single-molecule sequencing; transcriptional regulator |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Dina Grohmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Feb 2024 06:01 |
| Last Modified: | 22 Feb 2024 06:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58956 |
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