Bleckmann, Andrea and Spitzlberger, Nicole and Denninger, Philipp and Ehrnsberger, Hans F. and Wang, Lele and Bruckmann, Astrid and Reich, Stefan and Holzinger, Philipp and Medenbach, Jan and Grasser, Klaus D. and Dresselhaus, Thomas (2023) Cytosolic RGG RNA-binding proteins are temperature sensitive flowering time regulators in Arabidopsis. BIOLOGICAL CHEMISTRY, 404 (11-12). pp. 1069-1084. ISSN 1431-6730, 1437-4315
Full text not available from this repository. (Request a copy)Abstract
mRNA translation is tightly regulated by various classes of RNA-binding proteins (RBPs) during development and in response to changing environmental conditions. In this study, we characterize the arginine-glycine-glycine (RGG) motif containing RBP family of Arabidopsis thaliana representing homologues of the multifunctional translation regulators and ribosomal preservation factors Stm1 from yeast (ScStm1) and human SERBP1 (HsSERBP1). The Arabidopsis genome encodes three RGG proteins named AtRGGA, AtRGGB and AtRGGC. While AtRGGA is ubiquitously expressed, AtRGGB and AtRGGC are enriched in dividing cells. All AtRGGs localize almost exclusively to the cytoplasm and bind with high affinity to ssRNA, while being capable to interact with most nucleic acids, except dsRNA. A protein-interactome study shows that AtRGGs interact with ribosomal proteins and proteins involved in RNA processing and transport. In contrast to ScStm1, AtRGGs are enriched in ribosome-free fractions in polysome profiles, suggesting additional plant-specific functions. Mutant studies show that AtRGG proteins differentially regulate flowering time, with a distinct and complex temperature dependency for each AtRGG protein. In conclusion, we suggest that AtRGGs function in fine-tuning translation efficiency to control flowering time and potentially other developmental processes in response to environmental changes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; QUADRUPLEX; RIBOSOME; STM1P; TRANSLATION; AFFINITY; TRANSFORMATION; DELETION; DOMAIN; GENES; mRNA binding; RBP; RGG motif; ribosome; SERBP1; Stm1 |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Feb 2024 07:01 |
| Last Modified: | 22 Feb 2024 07:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/58984 |
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