Maisch, Tim and Scholz, Konstantin J. and Forster, Eva-Maria and Wenzl, Verena and Auer, David L. and Cieplik, Fabian and Hiller, Karl-Anton (2024) Optimal effective concentration combinations (OPECCs) for binary application of membrane-targeting antiseptics and TMPyP-mediated antimicrobial photodynamic therapy. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 23. pp. 189-196. ISSN 1474-905X, 1474-9092
Full text not available from this repository. (Request a copy)Abstract
The widespread occurrence of multi-resistant bacteria is a health problem of global dimension. Infections caused by multi-resistant pathogens are difficult to treat and often associated with high mortality. Therefore, new treatment strategies are of interest, such as the use of differently acting antibacterial concepts. One of these new concepts is the use of antiseptics in combination with the antibacterial photodynamic therapy (aPDT). Currently, no method has yet been established as a standard procedure for investigating combined effects and evaluating them in a generally valid and unambiguous manner. The focus of this study was on how cationic antiseptics benzalkonium chloride (BAC) and chlorhexidine digluconate (CHX) behave in a combined application with aPDT using the photosensitizer TMPyP. For this purpose, BAC and CHX were applied in combination with the aPDT using TMPyP in non-lethal concentrations to the three bacteria Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. The results of the combination experiments with sublethal concentrations of BAC or CHX with the aPDT showed that the binary application had a lethal effect. Irrespective of the bacteria, the reduction in concentrations in OPECC, compared to individual concentrations, was more than 50% for TMPyP, 23-40% for BAC, and 18-43% for CHX. Furthermore, the optimal effective concentration combinations (OPECCs) could be determined. The latter showed that the combined application allowed the reduction of both concentrations compared to the single application.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OUTER-MEMBRANE; PHOTOSENSITIZERS; INACTIVATION; ANTIBIOTICS; RESISTANCE; MECHANISMS; PORPHYRIN; TOXICITY; AUREUS; Antiseptics; Antimicrobial photodynamic therapy; aPDT; Binary; OPECC; Synergy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Feb 2024 07:28 |
| Last Modified: | 04 Mar 2025 10:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59008 |
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