Schroeder, Agnes and Oers, Talia and Byeon, Ye-Oun and Cieplik, Fabian and Proff, Peter and Kirschneck, Christian and Paddenberg, Eva (2023) Impact of Mechanical Strain and Nicotinamide on RUNX2-Deficient Osteoblast Mimicking Cleidocranial Dysplasia. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (23): 16581. ISSN 1661-6596, 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Cleidocranial dysplasia (CCD) is a rare genetic defect caused by a heterozygous mutation of runt-related transcription factor 2 (RUNX2), which is important for osteoblast and skeletal development. RUNX2-deficiency causes extra- and intra-oral malformations that often require orthodontic treatment. Nicotinamide (NAM) affects bone remodelling processes. As these are crucial for orthodontic therapy, NAM could improve orthodontic treatment in CCD patients. This study investigates the effect of NAM in control and RUNX2-deficient osteoblasts under mechanical strain mimicking orthodontic treatment. First, the optimal NAM concentration and the differences in the expression profile of control and RUNX2-deficient osteoblasts were determined. Subsequently, osteoblasts were exposed to tensile and compressive strain with and without NAM, and the expression of genes critically involved in bone remodelling was investigated. NAM increased the expression of bone remodelling genes. RUNX2-deficient osteoblasts expressed more receptor activator of NFkB ligand (RANKL) and interleukin-6 (IL6), but less colony-stimulating factor-1 (CSF1). Most of the positive effects of NAM on bone remodelling genes were impaired by mechanical loading. In conclusion, NAM stimulated osteoblast differentiation by increasing the expression of RUNX2 and regulated the expression of osteoclastogenic factors. However, the positive effects of NAM on bone metabolism were impaired by mechanical loading and RUNX2 deficiency.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DELAYED TOOTH ERUPTION; OSTEOCLAST DIFFERENTIATION; RECEPTOR ACTIVATOR; RUNX2; MUTATIONS; PATIENT; FORCES; GENE; IL-6; MICE; cleidocranial dysplasia (CCD); orthodontic tooth movement; mechanical strain; bone remodeling |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2024 14:24 |
| Last Modified: | 08 Mar 2024 14:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59192 |
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