Fleig, Claudia and Evert, Katja and Schlitt, Hans J. and Fichtner-Feigl, Stefan and Brunner, Stefan M. (2023) Phenotypic Characterization of Colorectal Liver Metastases: Capsule versus No Capsule and the Potential Role of Epithelial Mesenchymal Transition. CANCERS, 15 (4): 1056. ISSN , 2072-6694
Full text not available from this repository. (Request a copy)Abstract
Simple Summary Some colorectal liver metastases are surrounded by a fibrous capsule that has a positive impact on patient survival. This study aimed to characterize pathways that lead to this capsule formation. In colorectal liver metastases with capsule formation that consists of different collagen fibers, epithelial mesenchymal transition processes are upregulated and tumor cell migration is impaired. In summary, this leads to a less invasive tumor behavior when a capsule is present and can potentially be used to target tumor growth and spreading in the future. Background: Colorectal liver metastases (CRLM) can be encased in a fibrous capsule separating cancer from normal liver tissue, which correlates with increased patient survival. This study investigated the cellular and molecular components of capsule formation and the possible role of epithelial mesenchymal transition (EMT). Methods: From 222 patients with CRLM, 84 patients (37.8%) were categorized to have CRLM encased with a capsule. A total of 34 CRLM from 34 selected patients was analyzed in detail by EMT pathway-profiling and custom PCR arrays to identify differences in gene expression between CRLM with (n = 20) and without capsule (n = 14). In parallel, those 34 CRLM were used to analyze 16 gene products at the metastasis margin via immunohistochemistry. Results: Encapsulated CRLM showed an elevated expression of signal transduction pathways and effector molecules involved in EMT. E-cadherin and keratin-19 were more prevalent, and transcription as well as translation (immunohistochemistry) of pGSK-3-beta, SOX10, tomoregulin-1, and caldesmon were increased. By contrast, the loss of E-cadherin and the prevalence of snail-1 were increased in CRLM without capsule. Collagen I and III and versican were identified as capsule components with extracellular matrix fibers running concentrically around the malignant tissue and parallel to the invasive front. Caldesmon was also demonstrated as a capsule constituent. Conclusions: The fibrous capsule around CRLM can be produced by cells with mesenchymal characteristics. It functions as a protective border by both the features of fiber architecture and the inhibition of invasive growth through EMT recruiting mesenchymal cells such as myofibroblasts by transformation of surrounding epithelial or even carcinoma cells. By contrast, EMT demonstrated in non-encapsulated CRLM may lead to a more mesenchymal, mobile, and tissue-destructive carcinoma cell phenotype and facilitate malignant spread.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CANCER; EXPRESSION; VERSICAN; CALDESMON; GROWTH; PROGRESSION; FIBROSIS; COLLAGEN; TUMORS; fibrous capsule; EMT; capsule components; protective border |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Mar 2024 14:37 |
| Last Modified: | 08 Mar 2024 14:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59197 |
Actions (login required)
 |
View Item |
