Dengler, Linda and Meier, Julia and Klingl, Andreas and Nissl, Laura and Bellack, Annett and Grohmann, Dina and Rachel, Reinhard and Huber, Harald (2023) A novel interdomain consortium from a Costa Rican oil well composed of Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov. ARCHIVES OF MICROBIOLOGY, 205 (5): 189. ISSN 0302-8933, 1432-072X
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A novel interdomain consortium composed of a methanogenic Archaeon and a sulfate-reducing bacterium was isolated from a microbial biofilm in an oil well in Cahuita National Park, Costa Rica. Both organisms can be grown in pure culture or as stable co-culture. The methanogenic cells were non-motile rods producing CH4 exclusively from H-2/CO2. Cells of the sulfate-reducing partner were motile rods forming cell aggregates. They utilized hydrogen, lactate, formate, and pyruvate as electron donors. Electron acceptors were sulfate, thiosulfate, and sulfite. 16S rRNA sequencing revealed 99% gene sequence similarity of strain CaP3V-M-L2A(T) to Methanobacterium subterraneum and 98.5% of strain CaP3V-S-L1A(T) to Desulfomicrobium baculatum. Both strains grew from 20 to 42 degrees C, pH 5.0-7.5, and 0-4% NaCl. Based on our data, type strains CaP3V-M-L2A(T) (= DSM 113354( T) = JCM 39174( T)) and CaP3V-S-L1A(T) (= DSM 113299( T) = JCM 39179( T)) represent novel species which we name Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SULFATE-REDUCING BACTERIA; METHANOGENIC BACTERIA; RECLASSIFICATION; STRAIN; DEEP; Interdomain; Consortium; Biofilm; Methanogens; Sulfate-reducing bacteria |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Dina Grohmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2024 06:52 |
| Last Modified: | 01 Mar 2024 06:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59225 |
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