Dropco, Ivor and Philipp, Alois and Foltan, Maik and Lunz, Dirk and Lubnow, Matthias and Lehle, Karla (2023) Extended Cellular Deposits on Gas Exchange Capillaries Are Not an Indicator of Clot Formation: Analysis of Different Membrane Oxygenators. ASAIO JOURNAL, 69 (4). E134-E141. ISSN 1058-2916, 1538-943X
Full text not available from this repository. (Request a copy)Abstract
Antithrombogenic coatings of artificial surfaces within extracorporeal membrane oxygenation (ECMO) circuits improved its bio- and hemocompatibility. However, there is still a risk of thrombus formation in particular within the membrane oxygenator (MO). Since inflammatory cells are essential components within clots, the aim was to identify the extent of cellular accumulations on gas exchange capillaries from different ECMO systems. Thirty-four MOs (PLS, n = 27, Getinge; Hilite 7000 LT, n = 7, Fresenius Medical Care, Germany) were collected from adult patients. The extent of cellular deposits on gas exchange capillaries was classified using nuclear 4 ',6-diamidino-2-phenylindole staining and fluorescence microscopy. All Hilite oxygenators exhibited small cellular deposits. In contrast, the cellular distribution was heterogeneous on capillaries from PLS oxygenators: small deposits (34%), clusters (44%) and membrane-spanning cell structures (pseudomembranes) (22%). Overall, the median fluorescence intensity was significantly higher in the PLS group. Nevertheless, within 3 days before MO removal, there was no alteration in critical parameters (d-dimer and fibrinogen levels, platelet counts, and pressure drop across the MO). In conclusion, despite the histological differences on the gas capillaries from different types of oxygenators, there was no further evidence of increased inflammation and coagulation parameters that indicate clot formation within oxygenators.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXTRACORPOREAL LIFE-SUPPORT; FLOW; ANTICOAGULATION; CONTRIBUTES; THROMBOSIS; SYSTEMS; BLOOD; ECMO; biocompatibility; cell adhesion; indirect immunofluorescence; clot formation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Mar 2024 09:30 |
| Last Modified: | 09 Mar 2024 09:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59282 |
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