Burian, Marc and Velic, Ana and Matic, Katarina and Guenther, Stephanie and Kraft, Beatrice and Gonser, Lena and Forchhammer, Stephan and Tiffert, Yvonne and Naumer, Christian and Krohn, Michael and Berneburg, Mark and Yazdi, Amir S. and Macek, Boris and Schittek, Birgit (2015) Quantitative Proteomics of the Human Skin Secretome Reveal a Reduction in Immune Defense Mediators in Ectodermal Dysplasia Patients. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 135 (3). pp. 759-767. ISSN 0022-202X, 1523-1747
Full text not available from this repository. (Request a copy)Abstract
In healthy human skin host defense molecules such as antimicrobial peptides (AMPs) contribute to skin immune homeostasis. In patients with the congenital disease ectodermal dysplasia (ED) skin integrity is disturbed and as a result patients have recurrent skin infections. The disease is characterized by developmental abnormalities of ectodermal derivatives and absent or reduced sweating. We hypothesized that ED patients have a reduced skin immune defense because of the reduced ability to sweat. Therefore, we performed a label-free quantitative proteome analysis of wash solution of human skin from ED patients or healthy individuals. A clear-cut difference between both cohorts could be observed in cellular processes related to immunity and host defense. In line with the extensive underrepresentation of proteins of the immune system, dermcidin, a sweat-derived AMP, was reduced in its abundance in the skin secretome of ED patients. In contrast, proteins involved in metabolic/catabolic and biosynthetic processes were enriched in the skin secretome of ED patients. In summary, our proteome profiling provides insights into the actual situation of healthy versus diseased skin. The systematic reduction in immune system and defense-related proteins may contribute to the high susceptibility of ED patients to skin infections and altered skin colonization.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STAPHYLOCOCCUS-AUREUS INFECTION; ANTIMICROBIAL PEPTIDES; ECCRINE SWEAT; PROPIONIBACTERIUM-ACNES; ATOPIC-DERMATITIS; INNATE IMMUNITY; RNASE 7; EXPRESSION; DERMCIDIN; SEBOCYTES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Jul 2019 09:16 |
| Last Modified: | 24 Jul 2019 09:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5935 |
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