Wirth, Ulrike and Erl, Julia and Azzam, Saphia and Hoering, Carina and Skiba, Michael and Singh, Ritu and Hochmuth, Kathrin and Keller, Max and Wegener, Joachim and Koenig, Burkhard (2023) Monitoring the Reversibility of GPCR Signaling by Combining Photochromic Ligands with Label-free Impedance Analysis. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 62 (21). ISSN 1433-7851, 1521-3773
Full text not available from this repository. (Request a copy)Abstract
G protein-coupled cell surface receptors (GPCR) trigger complex intracellular signaling cascades upon agonist binding. Classic pharmacological assays provide information about binding affinities, activation or blockade at different stages of the signaling cascade, but real time dynamics and reversibility of these processes remain often disguised. We show that combining photochromic NPY receptor ligands, which can be toggled in their receptor activation ability by irradiation with light of different wavelengths, with whole cell label-free impedance assays allows observing the cell response to receptor activation and its reversibility over time. The concept demonstrated on NPY receptors may be well applicable to many other GPCRs providing a deeper insight into the time course of intracellular signaling processes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-COUPLED RECEPTORS; NEUROPEPTIDE-Y; ASSAYS; STIMULATION; BIOSENSOR; TARGETS; GPCR; Impedance Measurements; Label Free Detection; NPY Receptor; Photochromic Ligands |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Mar 2024 16:53 |
| Last Modified: | 09 Mar 2024 16:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59463 |
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