Markusch, Hanna and Michl-Holzinger, Philipp and Obermeyer, Simon and Thorbecke, Claudia and Bruckmann, Astrid and Babl, Sabrina and Laengst, Gernot and Osakabe, Akihisa and Berger, Frederic and Grasser, Klaus D. (2023) Elongation factor 1 is a component of the Arabidopsis RNA polymerase II elongation complex and associates with a subset of transcribed genes. NEW PHYTOLOGIST, 238 (1). pp. 113-124. ISSN 0028-646X, 1469-8137
Full text not available from this repository. (Request a copy)Abstract
Elongation factors modulate the efficiency of mRNA synthesis by RNA polymerase II (RNAPII) in the context of chromatin, thus contributing to implement proper gene expression programmes. The zinc-finger protein elongation factor 1 (ELF1) is a conserved transcript elongation factor (TEF), whose molecular function so far has not been studied in plants.Using biochemical approaches, we examined the interaction of Arabidopsis ELF1 with DNA and histones in vitro and with the RNAPII elongation complex in vivo. In addition, cytological assays demonstrated the nuclear localisation of the protein, and by means of double-mutant analyses, interplay with genes encoding other elongation factors was explored. The genome-wide distribution of ELF1 was addressed by chromatin immunoprecipitation.ELF1 isolated from Arabidopsis cells robustly copurified with RNAPII and various other elongation factors including SPT4-SPT5, SPT6, IWS1, FACT and PAF1C. Analysis of a CRISPR-Cas9-mediated gene editing mutant of ELF1 revealed distinct genetic interactions with mutants deficient in other elongation factors. Moreover, ELF1 associated with genomic regions actively transcribed by RNAPII. However, ELF1 occupied only c. 33% of the RNAPII transcribed loci with preference for inducible rather than constitutively expressed genes.Collectively, these results establish that Arabidopsis ELF1 shares several characteristic attributes with RNAPII TEFs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LEAF; DNA; TRIMETHYLATION; VERNALIZATION; METHYLATION; EXPRESSION; MECHANISM; INTERACTS; PROTEINS; BINDING; Arabidopsis thaliana; chromatin; gene activity; mRNA synthesis; RNA polymerase II; transcript elongation |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Mar 2024 17:41 |
| Last Modified: | 09 Mar 2024 17:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59493 |
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