Moehring, Steffen and Cieplik, Fabian and Hiller, Karl-Anton and Ebensberger, Helga and Ferstl, Gerlinde and Hermens, Joshua and Zaparty, Melanie and Witzgall, Ralph and Mansfeld, Ulrich and Buchalla, Wolfgang and Scholz, Konstantin Johannes (2023) Elemental Compositions of Enamel or Dentin in Human and Bovine Teeth Differ from Murine Teeth. MATERIALS, 16 (4): 1514. ISSN , 1996-1944
Full text not available from this repository. (Request a copy)Abstract
Teeth with different chemical compositions can show vastly different physical properties, so knowledge of elemental composition is required to use animal teeth as substitutes for human teeth in research. In vitro, energy dispersive X-ray spectroscopy (EDX), improved by calibration standards and Si3N4-window material, enables determining local elemental compositions of inorganic and organic compounds without sample destruction. Six human molars, bovine incisors, murine incisors, and murine molars were analyzed. EDX-field scans and line scans were analyzed for elements Ca, P, O, C, N, F, Na, Mg, Fe, Cl, and S. Furthermore, Ca/P- and Ca/N-Ratios were calculated. The presence of iron in murine incisor enamel was investigated using additional wavelength dispersive X-ray spectroscopy measurements (WDX) near the enamel surface. Bovine and human enamel and dentin revealed close similarities regarding elemental composition. The median (25-75% percentiles) of At%Ca was 21.1 (20.8-21.3) in human enamel, 21.0 (20.7-21.2) in bovine enamel, and in murine enamel, 18.3 (17.85-18.88) for molars and 18.35 (18.00-18.75) for incisors. In dentin, murine teeth revealed a higher At%Ca compared to human and bovine teeth. Significant differences between human and bovine teeth were found for nitrogen in dentin, with a median of 4.5 (3.3-5) At%N for human dentin and 2.7 (2.3-3.2) At%N for bovine dentin. The Ca/P-Ratio was the highest in human and bovine enamel, which did not differ significantly. Enamel from murine molars had a higher Ca/P-Ratio than murine incisors and the highest Ca/P-Ratio in dentin was observed for human teeth and murine molars (1.49). WDX revealed iron in the outer 10 mu m of pre- and post-eruptive enamel of murine incisors. Pre- and post-eruptive enamel on murine incisors only differed significantly in At%Ca (p = 0.041) and At%P (p = 0.026) with both At% higher in the pre-eruptive enamel. Murine teeth differ significantly from human and bovine teeth in terms of the elemental composition of enamel and dentin.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CARIES; MICROANALYSIS; SUBSTITUTE; TOOTH; MODEL; enamel; dentin; elemental composition; murine; bovine; human; EDX; WDX |
| Subjects: | 500 Science > 530 Physics 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Ralph Witzgall |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Mar 2024 10:25 |
| Last Modified: | 10 Mar 2024 10:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59537 |
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