Weigert, Nina and Schweiger, Anna-Lena and Gross, Jonas and Matthes, Marie and Corbacioglu, Selim and Sommer, Gunhild and Heise, Tilman (2023) Detection of a 7SL RNA-derived small non-coding RNA using Molecular Beacons in vitro and in cells. BIOLOGICAL CHEMISTRY, 404 (11-12). pp. 1123-1136. ISSN 1431-6730, 1437-4315
Full text not available from this repository. (Request a copy)Abstract
Small non-coding RNAs (sncRNA) are involved in many steps of the gene expression cascade and regulate processing and expression of mRNAs by the formation of ribonucleoprotein complexes (RNP) such as the RNA-induced silencing complex (RISC). By analyzing small RNA Seq data sets, we identified a sncRNA annotated as piR-hsa-1254, which is likely derived from the 3'-end of 7SL RNA2 (RN7SL2), herein referred to as snc7SL RNA. The 7SL RNA is an abundant long non-coding RNA polymerase III transcript and serves as structural component of the cytoplasmic signal recognition particle (SRP). To evaluate a potential functional role of snc7SL RNA, we aimed to define its cellular localization by live cell imaging. Therefore, a Molecular Beacon (MB)-based method was established to compare the subcellular localization of snc7SL RNA with its precursor 7SL RNA. We designed and characterized several MBs in vitro and tested those by live cell fluorescence microscopy. Using a multiplex approach, we show that 7SL RNA localizes mainly to the endoplasmic reticulum (ER), as expected for the SRP, whereas snc7SL RNA predominately localizes to the nucleus. This finding suggests a fundamentally different function of 7SL RNA and its derivate snc7SL RNA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESSENGER-RNA; LA; PHOSPHORYLATION; EXPRESSION; FRAGMENTS; TRANSPORT; ANTIGEN; LOOP; sncRNA; molecular beacon; piRNA; live cell; imaging; fluorescence |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Pädiatrische Hämatologie, Onkologie und Stammzelltransplantation |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Mar 2024 14:00 |
| Last Modified: | 12 Mar 2024 07:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59595 |
Actions (login required)
 |
View Item |
