Kanis, Franziska M. and Meier, Johannes P. and Guldan, Harald and Niller, Hans-Helmut and Dahm, Michael and Dansard, Alexander and Zander, Thomas and Struck, Friedhelm and Soutschek, Erwin and Deml, Ludwig and Moebus, Selina and Barabas, Sascha (2023) Performance of T-Track® SARS-CoV-2, an Innovative Dual Marker RT-qPCR-Based Whole-Blood Assay for the Detection of SARS-CoV-2-Reactive T Cells. DIAGNOSTICS, 13 (17): 2722. ISSN , 2075-4418
Full text not available from this repository. (Request a copy)Abstract
T-cell immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a central role in the control of the virus. In this study, we evaluated the performance of T-Track (R) SARS-CoV-2, a novel CE-marked quantitative reverse transcription-polymerase chain reaction (RT-qPCR) assay, which relies on the combined evaluation of IFNG and CXCL10 mRNA levels in response to the S1 and NP SARS-CoV-2 antigens, in 335 participants with or without a history of SARS-CoV-2 infection and vaccination, respectively. Of the 62 convalescent donors, 100% responded to S1 and 88.7% to NP antigens. In comparison, of the 68 naive donors, 4.4% were reactive to S1 and 19.1% to NP. Convalescent donors <50 and =50 years of age demonstrated a 100% S1 reactivity and an 89.1% and 87.5% NP reactivity, respectively. T-cell responses by T-Track (R) SARS-CoV-2 and IgG serology by recomLine SARS-CoV-2 IgG according to the time from the last immunisation (by vaccination or viral infection) were comparable. Both assays showed a persistent cellular and humoral response for at least 36 weeks post immunisation in vaccinated and convalescent donors. Our results demonstrate the very good performance of the T-Track (R) SARS-CoV-2 molecular assay and suggest that it might be suitable to monitor the SARS-CoV-2-specific T-cell response in COVID-19 vaccinations trials and cross-reactivity studies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COVID-19; RESPONSES; IMMUNITY; MEMORY; T-Track((R)) SARS-CoV-2; COVID-19; T cells; T-cell immunity; RT-qPCR; CXCL10; IFNG; SARS-CoV-2; S1 antigen; NP antigen |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Mar 2024 11:02 |
| Last Modified: | 12 Mar 2024 11:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59732 |
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