Ronca, Alfredo and D'Amora, Ugo and Capuana, Elisa and Zihlmann, Carla and Stiefel, Niklaus and Pattappa, Girish and Schewior, Ruth and Docheva, Denitsa and Angele, Peter and Ambrosio, Luigi (2023) Development of a highly concentrated collagen ink for the creation of a 3D printed meniscus. HELIYON, 9 (12): e23107. ISSN , 2405-8440
Full text not available from this repository. (Request a copy)Abstract
The most prevalent extracellular matrix (ECM) protein in the meniscus is collagen, which controls cell activity and aids in preserving the biological and structural integrity of the ECM. To create stable and high-precision 3D printed collagen scaffolds, ink formulations must possess good printability and cytocompatibility. This study aims to overlap the limitation in the 3D printing of pure collagen, and to develop a highly concentrated collagen ink for meniscus fabrication. The extrusion test revealed that 12.5 % collagen ink had the best combination of high collagen concentration and printability. The ink was specifically designed to have load-bearing capacity upon printing and characterized with respect to rheological and extrusion properties. Following printing of structures with different infill, a series of post-processing steps, including salt stabilization, pH shifting, washing, freeze-drying, crosslinking and sterilization were performed, and optimised to maintain the stability of the engineered construct. Mechanical testing highlighted a storage modulus of 70 kPa for the lower porous structure while swelling properties showed swelling ratio between 9 and 11 after 15 min of soaking. Moreover, human avascular and vascular meniscus cells cultured on the scaffolds deposited a meniscus-like matrix containing collagen I, II and glycosaminoglycans after 28 days of culture. Finally, as proof-of-concept, human size 3D printed meniscus scaffold were created.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SCAFFOLDS; FABRICATION; EXTRUSION; HYDROGEL; Collagen; 3D printing; Meniscus tissue engineering; Vascular and avascular regions |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Jan 2024 09:50 |
| Last Modified: | 30 Jan 2024 09:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59829 |
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